Immortality

Immortality is a magical ability to live forever.

It featured on ABC's Once Upon a Time. It first appears in the "Pilot" of the Season 1.

Description
Immortality is one of the magical abilities that the Holy Grail grants to the person who drinks from it. After Nimue corrupts herself and her Holy Grail magic, and becomes the first Dark One, this ability still remains in her and all the other Dark Ones. ("Nimue (episode)")

But as Mr. Gold says, immortality doesn't mean one can't be killed, but means to live forever. It is proven when Hook stabbed Mr. Gold with the deadly poison Dreamshade in the Land Without Magic and Mr. Gold had no option but to die unless he somehow exchanges someone else's life for his. ("The Queen Is Dead", "The Miller's Daughter", "Second Star to the Right")

The Dark One's Dagger is also the main and most-known item that can kill any current Dark One, and that's why, it is said that the dagger is Dark Ones' weakness numerous times. And because the dagger is originally part of Excalibur, the blade also has the ability to kill any Dark One. ("Desperate Souls", " The Return", " Swan Song")

It is possible to hurt a Dark One with a simple sword and make them bleed, but it cannot kill them. Also when Robin Hood shoots an arrow to Rumplestiltskin, he simply removes the arrow from his chest and continues to live like nothing ever happened. ("A Land Without Magic", " Lacey")

Immortal or not, there is no escape from the "Worse Place". Soul ripping arrows can also be used as an alternate way. ("Her Handsome Hero")

According to the Evil Queen from the Wish Realm, Golden Flowers also have the ability to kill an immortal person. ("Eloise Gardener")

Deities also have this ability, as they are the ones who gifted the Holy Grail to mankind in the first place. but much like the Dark Ones, Deities also have their own "weakness" called the Olympian Crystal, which can, not just kill, but erase their existance. ("Last Rites")

There are also immortal people who have magic like Maleficent, or creatures like Medusa. However, how they got this gift or why they have it is unknown.

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